CrisprLens

CrisprLens

Guide RNA efficiency and off-target risk prediction

gRNA Sequence 23-mer · A T G C only · last 3 = PAM

DNA Target optional provide for paired scoring · leave empty for auto-scan

On-Target Efficiency

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Off-Target Risk

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Candidate Off-Target Sites

DNA Sequence	Mismatches	Raw Score	Relative Rank

Position Saliency

Integrated Gradients · on-target head · seed region positions 12–20